Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus
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منابع مشابه
Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus
Most mushrooms are thermo-sensitive to temperatures over 23°C, which greatly restricts their agricultural cultivation. Understanding mushroom's innate heat-tolerance mechanisms may facilitate genetic improvements of their thermotolerance. Agaricus bisporus strain 02 is a relatively thermotolerant mushroom strain, while strain 8213 is quite thermo-sensitive. Here, we compared their responses at ...
متن کاملCloning, expression, and characterization of para-aminobenzoic acid (PABA) synthase from Agaricus bisporus 02, a thermotolerant mushroom strain.
The pabS gene of Agaricus bisporus 02 encoding a putative PABA synthase was cloned, and then the recombinant protein was expressed in Escherichia coli BL21 under the control of the T7 promoter. The enzyme with an N-terminal GST tag or His tag, designated GST-AbADCS or His-AbADCS, was purified with glutathione Sepharose 4B or Ni Sepharose 6 Fast Flow. The enzyme was an aminodeoxychorismate synth...
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To characterize thermotolerance-related genes in Agaricus bisporus strain 02, we employed differential display PCR (DD-PCR) to analyze total RNA samples extracted from the mycelia grown at different temperatures. Two partial DNA fragments (023-11A and 023-11B) were cloned thus far, the expression of which was correlated with the culturing temperature. The sequences of the two DNA fragments were...
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The symptoms of viral infections of fungi range from cryptic to severe, but there is little knowledge of the factors involved in this transition of fungal/viral interactions. Brown cap mushroom disease of the cultivated Agaricus bisporus is economically important and represents a model system to describe this transition. Differentially expressed transcript fragments between mushrooms showing th...
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The culmination of the first half-century of investigations into the genome of Agaricus bisporus was marked by the first whole-genome sequencing for a strain of this species. The genome of a second strain of A. bisporus was sequenced the following year. While a structural view of the genome is now effectively complete, functional interpretations of the many gene sequences, supported by accumula...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0091298